e coli β lactamase  (Databank Inc)

Journal: RSC Advances

Article Title: Development of multifunctional magnetic core–shell manganese TiO 2 photocatalysts for sustainable wastewater treatment: synergistic dye mineralization, antibacterial activity, and molecular docking insights

doi: 10.1039/d6ra00672h

Figure Lengend Snippet: Percent log reduction using FeMnT (a) dopant concentration (0.1–5 mol%) and calcination temperatures (300 °C and 500 °C) (b) different pathogens ( E. coli and S. aureus ) (c) determination of Minimum Bactericidal Concentration (MBC) (d) kill time analysis against S. aureus using control, Bare TiO 2 and 0.75FeMnT calcined at different calcination temperatures (300 °C and 500 °C) against E. coli and S. aureus .

Article Snippet: A three-dimensional structure of E. coli β-lactamase (PDB ID: 1ERM, (68) resolution = 1.70 Å) in complex with boronic acid inhibitor (1 R )-1-acetamido-2-(3-carboxyphenyl)ethane boronic acid was taken from Protein Databank.

Techniques: Concentration Assay, Control

Journal: RSC Advances

Article Title: Development of multifunctional magnetic core–shell manganese TiO 2 photocatalysts for sustainable wastewater treatment: synergistic dye mineralization, antibacterial activity, and molecular docking insights

doi: 10.1039/d6ra00672h

Figure Lengend Snippet: Photocatalyst recycling studies using 0.75 FeMnT-3 against (a) RY145 photodegradation and (b) tested bacterial pathogens S. aureus and E. coli .

Article Snippet: A three-dimensional structure of E. coli β-lactamase (PDB ID: 1ERM, (68) resolution = 1.70 Å) in complex with boronic acid inhibitor (1 R )-1-acetamido-2-(3-carboxyphenyl)ethane boronic acid was taken from Protein Databank.

Techniques:

Journal: RSC Advances

Article Title: Development of multifunctional magnetic core–shell manganese TiO 2 photocatalysts for sustainable wastewater treatment: synergistic dye mineralization, antibacterial activity, and molecular docking insights

doi: 10.1039/d6ra00672h

Figure Lengend Snippet: The binding mode of the photocatalyst (represented by the cyan ball and sticks) is shown in the active site of β-lactamase enzyme (shown as yellow ribbons). The interactions with the active site residues are highlighted in the box, where bonds are depicted in black dashed lines, while solvent–ligand interaction is shown in green line.

Article Snippet: A three-dimensional structure of E. coli β-lactamase (PDB ID: 1ERM, (68) resolution = 1.70 Å) in complex with boronic acid inhibitor (1 R )-1-acetamido-2-(3-carboxyphenyl)ethane boronic acid was taken from Protein Databank.

Techniques: Binding Assay, Solvent

Journal: Nature

Article Title: Commensal consortia decolonize Enterobacteriaceae via ecological control

doi: 10.1038/s41586-024-07960-6

Figure Lengend Snippet: a , GF B6 mice ( n = 3–10 per group) were monocolonized with the indicated pathogenic or antibiotic-resistant strain, and then treated with the indicated bacterial mixture. Faecal pathobiont load was examined by counting CFUs or by qPCR of bacterial DNA (for C. difficile ). b , c , GF B6 mice were colonized with faecal microbiota from a patient with Crohn’s disease (CD15) containing a high level of K. pneumoniae ( b ) or from a patient with ulcerative colitis (UC5) containing ESBL + E. coli ( c ). All mice were subsequently treated with vancomycin (VCM), and half of the mice received oral F18-mix administration four times over the next two days. Full-length 16S rRNA gene sequencing was performed on faecal samples to determine the relative abundance of detected strains. d – f , GF Il10 −/− mice ( n = 6 per group) were colonized with UC5 microbiota and then treated with F18-mix, F13-mix or vehicle control; faecal CFUs of ESBL + E. coli throughout the experiment ( d ), representative haematoxylin and eosin staining of the colon on day 28 ( e ; scale bars, 200 μm) and histological colitis scores on day 28 ( f ) are shown. Data in a , d , f , are median ± IQR and are compared by Kruskal–Wallis test using the Benjamini–Hochberg correction for multiple comparisons.

Article Snippet: To examine the effects of defined consortia on pathogenic bacteria, C57BL/6 GF mice (8–14 weeks of age, housed in separate GF isolators) were inoculated with K. pneumoniae 2H7 (Kp-2H7), carbapenem-resistant K. pneumoniae (CPM + Kp, ATCC BAA1705), K. aerogenes (strain Ka-11E12 ), extended-spectrum-β-lactamase producing E. coli (ESBL + E. coli , ATCC BAA2777), adherent-invasive E. coli (AIEC, strain LF82, provided by N. Barnich ), P. aeruginosa (ATCC 10145), vancomycin-resistant E. faecium (VRE Ef, ATCC 700221), C. upsaliensis (ATCC BAA1059), or C. difficile (strain 630, ATCC BAA1382) by oral gavage (2 × 10 8 CFU per mouse).

Techniques: Sequencing, Control, Staining

Journal: Nature

Article Title: Commensal consortia decolonize Enterobacteriaceae via ecological control

doi: 10.1038/s41586-024-07960-6

Figure Lengend Snippet: a, b , GF B6 mice were colonized with faecal microbiota from either a patient with Crohn’s disease (CD#15) containing a high level of K. pneumoniae ( a ) or from a patient with ulcerative colitis (UC#5) containing ESBL + E .coli ( b ). All mice were subsequently treated with vancomycin, and half received oral F18-mix administration four times over two days. CFUs of K. pneumoniae and E. coli (upper panels) and Shannon index (lower panels) of the faecal microbiota were examined longitudinally and compared by Mann-Whitney U test at day 28 (two-sided). c-g , GF Il10 −/− mice were monocolonized with Kp-2H7, then orally administered the indicated bacterial mix seven days later. Representative haematoxylin and eosin staining of the colon (scale bar = 100 μm) ( d ), histological colitis scores ( e ), faecal lipocalin-2 and calprotectin levels ( f ), and frequency of IFNγ + cells among colonic lamina propria CD4 + TCRβ + T cells ( g ) are shown. In panels a - c and e - g , median ± IQR are shown, representative of two independent experiments. Statistical analysis was performed using the Kruskal-Wallis test with the Benjamini-Hochberg correction for multiple comparisons.

Article Snippet: To examine the effects of defined consortia on pathogenic bacteria, C57BL/6 GF mice (8–14 weeks of age, housed in separate GF isolators) were inoculated with K. pneumoniae 2H7 (Kp-2H7), carbapenem-resistant K. pneumoniae (CPM + Kp, ATCC BAA1705), K. aerogenes (strain Ka-11E12 ), extended-spectrum-β-lactamase producing E. coli (ESBL + E. coli , ATCC BAA2777), adherent-invasive E. coli (AIEC, strain LF82, provided by N. Barnich ), P. aeruginosa (ATCC 10145), vancomycin-resistant E. faecium (VRE Ef, ATCC 700221), C. upsaliensis (ATCC BAA1059), or C. difficile (strain 630, ATCC BAA1382) by oral gavage (2 × 10 8 CFU per mouse).

Techniques: MANN-WHITNEY, Staining

Journal: Nature

Article Title: Commensal consortia decolonize Enterobacteriaceae via ecological control

doi: 10.1038/s41586-024-07960-6

Figure Lengend Snippet: Bacterial strains isolated from donors F, K, or I were cultured in mGAM broth containing 300 μM gluconate for 48 hr at 37 °C (n = 3 biological replicates). Gluconate concentration in the culture supernatant was measured by LC-MS/MS and is depicted in the middle bar graph. Data are shown as median ± IQR. Genomes of cultured strains were sequenced and examined for carriage of genes putatively involved in gluconate metabolism. For classical pathway genes, gluconate kinase ( gntK , MKMCEHOJ_02531) and gluconate transporter sequences (MKMCEHOJ_02530, MKMCEHOJ_02505) from the f37_ E. coli strain were used as the reference. For alternative pathway genes, gluconate dehydratase ( gad , EAOGLLOI_00767), gluconate transporter sequences (EAOGLLOI_00766, EAOGLLOI_00912), 2-dehydro-3-deoxygluconokinase ( kdgK , EAOGLLOI_00768), and 2-dehydro-3-deoxyphosphogluconate aldolase ( eda , EAOGLLOI_00769) from the f17_ Blautia caecimuris strain were used as the reference. Asterisk indicates that the gluconate dehydratase in the f19_ Blautia caecimuris strain is nonfunctional due to a frameshift mutation. GntK, gluconate kinase. GAD, gluconate dehydratase.

Article Snippet: To examine the effects of defined consortia on pathogenic bacteria, C57BL/6 GF mice (8–14 weeks of age, housed in separate GF isolators) were inoculated with K. pneumoniae 2H7 (Kp-2H7), carbapenem-resistant K. pneumoniae (CPM + Kp, ATCC BAA1705), K. aerogenes (strain Ka-11E12 ), extended-spectrum-β-lactamase producing E. coli (ESBL + E. coli , ATCC BAA2777), adherent-invasive E. coli (AIEC, strain LF82, provided by N. Barnich ), P. aeruginosa (ATCC 10145), vancomycin-resistant E. faecium (VRE Ef, ATCC 700221), C. upsaliensis (ATCC BAA1059), or C. difficile (strain 630, ATCC BAA1382) by oral gavage (2 × 10 8 CFU per mouse).

Techniques: Isolation, Cell Culture, Concentration Assay, Liquid Chromatography with Mass Spectroscopy, Mutagenesis